Prof. Dr. rer. nat. Reinhold Schäfer
Senior Professor at the Charité Comprehensive Cancer Center (CCCC)
Molecular Tumor Biology
Charité - Universitätsmedizin Berlin
Email: reinhold.schaefer@charite.de
Phone: 030 450 564 640
University Education
- 1976 Ph.D., Universität Bonn
Professional Experience (selection)
- 1976-1978 Postdoctoral fellow at the Division of Molecular Genetics, Max Planck Institute for Experimental Medicine (MPI), Göttingen
- 1978-1985 Postdoctoral fellow at the Institute of Cell Biology (Tumor Research), West German Cancer Center, Universität Essen
- 1985-1988 Principal investigator, Ludwig Institute for Cancer Research, Bern, Switzerland
- 1988-1996 Head, Division of Cancer Research, Department of Pathology, University Hospital Zürich, Switzerland
- 1996-2008 Professor of Molecular Tumor Pathology, Institute of Pathology, Charité
- 2009-2018 Deputy director (Translational Research), CCCC
- 2019- Senior Professor at CCCC
Most important Awards, Grants or Scientific Achievements
- 1988 Swiss Cancer Award (Robert-Wenner-Preis), Swiss Cancer Society
- 2001 Award of the Stifterverband für die deutsche Wissenschaft
Scientific Scope
General interest:
Understanding the global genome-wide effects of RAS oncogenes on cellular transformation, tumor formation and therapy resistance.
Specific research themes:
1) Investigation of alterations of the genetic program mediated by mutant RAS genes in generic cell culture models, patient tumor-derived organoid cultures by means of multi-omics approaches.
2) Identification of specific vulnerabilities of cells expressing RAS mutants and exploiting their relevance for targeted therapy.
3) Identification of small molecular weight compounds able to block the activity of RAS pathway-responsive transcription factors in solid tumor models (colorectal cancer, pancreatic cancer, melanoma).
4) Characterization of transcription factor networks that execute transformed properties and phenotypes mediated by chronic activation of the RAS signaling system.
5) Translation of preclinical evidence obtained in model systems into early clinical trials.
Future directions
Validation of drug response predictions obtained in generic models in patient tumor-derived organoid cultures (PDO) and xenotransplants (PDX). Analysis of the downstream effects of targeted intervention at the systems level. Provision of a mechanistic understanding of drug sensitivities and resistance.
Selected references
- Schütte, M., Risch, T., Abdavi-Azar, N., Boehnke, K., Schumacher, D., Keil, M., Yildiriman, R., Jandrasits, C., Borodina, T., Amstislavskiy, V., Worth, C. L., Schweiger, C., Liebs, S., Lange, M., Warnatz, H. J., Butcher, L. M., Barrett, J. E., Sultan, M., Wierling, C., Golob-Schwarzl, N., … Yaspo, M. L. (2017). Molecular dissection of colorectal cancer in pre-clinical models identifies biomarkers predicting sensitivity to EGFR inhibitors. Nature communications, 8, 14262.
- Pallarz, S., Benary, M., Lamping, M., Rieke, D., Starlinger, J., Sers, C., Wiegandt, D. L., Seibert, M., Ševa, J., Schäfer, R., Keilholz, U., & Leser, U. (2019). Comparative Analysis of Public Knowledge Bases for Precision Oncology. JCO precision oncology, 3, PO.18.00371.
- Kulbe, H., Otto, R., Darb-Esfahani, S., Lammert, H., Abobaker, S., Welsch, G., Chekerov, R., Schäfer, R., Dragun, D., Hummel, M., Leser, U., Sehouli, J., & Braicu, E. I. (2019). Discovery and Validation of Novel Biomarkers for Detection of Epithelial Ovarian Cancer. Cells, 8(7), 713.
- Gastl, B., Klotz-Noack, K., Klinger, B., Ispasanie, S., Salib, K., Zuber, J., Mamlouk, S., Bublitz, N., Blüthgen, N., Horst, D., Morkel, M., Schäfer, R., & Sers, C. (2020). Reduced replication origin licensing selectively kills KRAS-mutant colorectal cancer cells via mitotic catastrophe. Cell death & disease, 11(7), 499.
- Mamlouk, S., Childs, L. H., Aust, D., Heim, D., Melching, F., Oliveira, C., Wolf, T., Durek, P., Schumacher, D., Bläker, H., von Winterfeld, M., Gastl, B., Möhr, K., Menne, A., Zeugner, S., Redmer, T., Lenze, D., Tierling, S., Möbs, M., Weichert, W., … Sers, C. (2017). DNA copy number changes define spatial patterns of heterogeneity in colorectal cancer. Nature communications, 8, 14093.
- Wolkenhauer, O., Auffray, C., Baltrusch, S., Blüthgen, N., Byrne, H., Cascante, M., Ciliberto, A., Dale, T., Drasdo, D., Fell, D., Ferrell, J. E., Jr, Gallahan, D., Gatenby, R., Günther, U., Harms, B. D., Herzel, H., Junghanss, C., Kunz, M., van Leeuwen, I., Lenormand, P., … Jaster, R. (2010). Systems biologists seek fuller integration of systems biology approaches in new cancer research programs. Cancer research, 70(1), 12–13.
- Truss, M., Swat, M., Kielbasa, S. M., Schäfer, R., Herzel, H., & Hagemeier, C. (2005). HuSiDa--the human siRNA database: an open-access database for published functional siRNA sequences and technical details of efficient transfer into recipient cells. Nucleic acids research, 33(Database issue), D108–D111.
- Regan, J. L., Schumacher, D., Staudte, S., Steffen, A., Haybaeck, J., Keilholz, U., Schweiger, C., Golob-Schwarzl, N., Mumberg, D., Henderson, D., Lehrach, H., Regenbrecht, C., Schäfer, R., & Lange, M. (2017). Non-Canonical Hedgehog Signaling Is a Positive Regulator of the WNT Pathway and Is Required for the Survival of Colon Cancer Stem Cells. Cell reports, 21(10), 2813–2828.
- Schumacher, D., Andrieux, G., Boehnke, K., Keil, M., Silvestri, A., Silvestrov, M., Keilholz, U., Haybaeck, J., Erdmann, G., Sachse, C., Templin, M., Hoffmann, J., Boerries, M., Schäfer, R., & Regenbrecht, C. (2019). Heterogeneous pathway activation and drug response modelled in colorectal-tumor-derived 3D cultures. PLoS genetics, 15(3), e1008076.
- Stelniec-Klotz, I., Legewie, S., Tchernitsa, O., Witzel, F., Klinger, B., Sers, C., Herzel, H., Blüthgen, N., & Schäfer, R. (2012). Reverse engineering a hierarchical regulatory network downstream of oncogenic KRAS. Molecular systems biology, 8, 601.